Infection control is an essential component of health care settings such as hospitals, clinics, and laboratories. Infection control measures can be as easy as washing hands and as complicated as high-level disinfection of surgical instruments in a healthcare setting. Implementing infection control measures can prevent transmission of diseases in healthcare settings and the community. Sterilization is a component of the infection control procedure. It is a process to remove or destroy infectious agents such as bacteria, fungi, and viruses from materials or surfaces. All reusable items that come in contact with the patient’s blood, saliva, or mucous membranes must be sterilized to prevent infection. To ensure minimal instrument damage during sterilization, microorganisms are killed by oxidation, disruption of cell membranes, interruption of DNA synthesis, and interference with protein synthesis. To sterilize an instrument three definite stages: pre-sterilization cleaning
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